Impact of DNA mutations on lifelong blood cell production uncovered  – Innovita Research

Impact of DNA mutations on lifelong blood cell production uncovered 

Researchers discover how leukemia-associated gene mutations steadily commandeer blood cell production over a lifetime, and how these changes relate to aging and cancer development.

Image credit: Pixabay

New research has uncovered how genetic mutations hijack the production of blood cells in different periods of life. Scientists at the Wellcome Sanger Institute, the Cambridge Stem Cell Institute, EMBL’s European Bioinformatics Institute (EMBL-EBI), and collaborators show how these changes relate to aging and the development of age-related diseases, including blood cancer.

The new study, published in Nature, represents the first time that the lifelong impact of genetic mutations on cell growth dynamics has been explored.

All human cells acquire genetic changes in their DNA throughout life, known as somatic mutations, with a specific subset of mutations driving cells to multiply. This is common in professional blood-making cells, known as blood stem cells, and results in the growth of populations of cells with identical mutations known as ‘clones’. This process, termed ‘clonal hematopoiesis’, becomes ubiquitous with age and is a risk factor for developing blood cancer and other age-related conditions.

To understand how and when clonal hematopoiesis develops, how it is influenced by aging, and how it relates to disease, the researchers tracked nearly 700 blood cell clones from 385 individuals aged over 55, who were part of the SardiNIA longitudinal study1. Participants donated regular blood samples for up to 16 years.

DNA sequencing of blood samples showed that 92.4 percent of clones expanded at a stable exponential rate over the period studied. The rate of growth was primarily influenced by the nature of the mutated gene in each clone.

After capturing the behavior of clones in later life, the team used mathematical models to infer their growth patterns over the entire human lifespan. They uncovered that clone behavior changed dramatically with age depending on the identity of the mutated gene.

First, clones driven by mutations in DNMT3A, expanded fast in young people and then decelerated in old age. Second, clones driven by mutations in TET2 appeared and grew uniformly throughout life, such that they became more common than DNMT3A-mutant clones after the age of 75. Finally, clones with mutations in splicing genes, U2AF1, and SRSF2, only expanded exclusively later in life and exhibited some of the fastest growth.

These age-dependent clonal behaviors mirror the frequency of emergence of different types of blood cancers and reveal that mutations associated with fast clonal growth are more likely to lead to malignancy.

Source: Sanger Institute